In Vivo Characterization of a Red Light-Activated Vasodilation: A Photobiomodulation Study

• DOI: 10.3389/fphys.2022.880158
• Publication Date: 2022-05-02T05:50:10Z
• Authors: Agnes Keszler; Brian Lindemer; Grant Broeckel; Dorothee Weihrauch; Yan Gao; Nicole L. Lohr
• Keywords: Characterization

Nitric oxide dependent vasodilation is an effective mechanism for restoring blood flow to ischemic tissues. Previously, we established ex vivo murine model whereby red light (670 nm) facilitates via endothelium derived vasoactive species which contains a functional group that can be reduced nitric oxide. In the present study investigated this vasodilator in by measuring with Laser Doppler Perfusion imaging mice. The vasodilatory precursor was analyzed plasma and muscle triiodide-dependent chemiluminescence. First, 5–10 min irradiation of 3 cm 2 area hind limb at 670 nm (50 mW/cm ) produced optimal vasodilation. irradiated quadriceps tissue decreased significantly from 123 ± 18 pmol/g both intensity duration treatment average 90 17 tissue, while stayed steady (137 21 tissue) unexposed control hindlimb. Second, remained elevated 30 after termination exposure. content increased 50% then depleted plasma, stable hindlimb muscle. Third, mimic human peripheral artery disease, ameroid constrictor inserted on proximal femoral mice caused significant reduction flow. Repeated 14 days achieved increase perfusion constricted limb. Our results strongly support regulate dilation conduit vessel releasing may offer simple home-based therapy future individuals impaired leg.