TWIK-Related Acid-Sensitive Potassium Channels (TASK-1) Emerge as Contributors to Tone Regulation in Renal Arteries at Alkaline pH

• DOI: 10.3389/fphys.2022.895863
• Publication Date: 2022-05-20T07:08:57Z
• Authors: Anastasia A. Shve...; Varvara S. Lazarenko; Dina K. Gaynullina; Olga S. Tarasova; Rudolf Schubert
• Keywords: Vascular tone; Tone (literature)

Aim: TASK-1 channels are established regulators of pulmonary artery tone but their contribution to the regulation vascular in systemic arteries is poorly understood. We tested hypothesis that channel functional impact differs among beds, this associated with differences expression and may increase alkalization extracellular environment. Therefore, we evaluated level vasomotor role mesenteric renal arteries. Methods: Pulmonary, from male Wistar rats were used for mRNA (qPCR) protein content (Western blotting) measurements. The was studied by wire myography using blocker AVE1231. In some experiments, endothelium removed a rat whisker. Results: Expression levels both pore-forming subunit highest arteries, lowest had an intermediate value Blockade 1 µM AVE1231 increased U46619-induced contractile responses did not affect basal methoxamine at physiological pH (pHo = 7.41). At alkaline 7.75 (increase NaHCO 3 52 mM) evoked development low concentrations This effect independent endothelium. Conclusion: circulation, abundant have anticontractile function under conditions alkalosis.