Development of CRISPR/Cas9-Mediated Gene-Drive Construct Targeting the Phenotypic Gene in Plutella xylostella

• DOI: 10.3389/fphys.2022.938621
• Publication Date: 2022-06-29T07:01:56Z
• Authors: Muhammad Asad; Dan Liu; Jianwen Li; Jing Chen; Guang Yang
• Keywords: Gene drive

The gene-drive system can ensure that desirable traits are transmitted to the progeny more than normal Mendelian segregation. clustered regularly interspersed palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) mediated has been demonstrated in dipteran insect species, including Drosophila and Anopheles, not yet other species. Here, we have developed a single CRISPR/Cas9-mediated construct for Plutella xylostella, highly-destructive lepidopteran pest of cruciferous crops. was containing Cas9 gene, marker gene (EGFP) gRNA sequence targeting phenotypic (Pxyellow) site-specifically inserted into P. xylostella genome. This homing-based copied ∼12 kb fragment gRNA, EGFP along with their promoters target site. Overall, 6.67%-12.59% efficiency due homology-directed repair (HDR), 80.93%-86.77% resistant-allele formation non-homologous-end joining (NHEJ) were observed. Furthermore, transgenic derived from male parents showed higher compared female parents. study demonstrates feasibility inherits desired progeny. finding this provides foundation develop an effective control.