Hypertrophy of the right ventricle by pulmonary artery banding in rats: a study of structural, functional, and transcriptomics alterations in the right and left ventricles

• DOI: 10.3389/fphys.2023.1129333
• Publication Date: 2023-07-27T21:14:27Z
• Authors: Jairo Montemor Au...; Ednei Luiz Antonio; Luis Felipe Neves...; Andrey Jorge Serra; Regiane Santos Fe...; Jose Antonio Silv...; Silvia Saiuli Mik...; Paulo Jose Ferrei...; Valdir Ambrosio M...
• Keywords: Right ventricular hypertrophy; Left Ventricles; Right pulmonary artery; Left and right; Right heart

Introduction: Right ventricular remodeling with subsequent functional impairment can occur in some clinical conditions adults and children. The triggering factors, molecular mechanisms, and, especially, the evolution over time are still not well known. Left (LV) changes associated right (RV) also poorly understood. Objectives: study aimed to evaluate RV morphological, functional, gene expression parameters rats submitted pulmonary artery banding compared control rats, temporal of these parameters, analyze influence by their controls on LV geometry, histology, expression, performance. Methods: Healthy 6-week-old male Wistar-EPM weighing 170–200 g were included. One day after echocardiogram, depending animals undergoing ( PAB) procedure or (control group), they then randomly divided into subgroups according follow-up time: 72 h, 2, 4, 6, 8 weeks. In each subgroup, following conducted: a new hemodynamic study, collection material for morphological analysis (hypertrophy fibrosis), biology (gene expression). results presented as mean ± standard deviation mean. A two-way ANOVA Tukey post-test variables times. adopted significance level was 5%. Results: There no significant difference among percentage water both lungs liver (the ranged from 76% 78% that 67% 71%). weight chambers significantly higher PAB all (RV weighed 0.34 0.48 g, subjects, 0.17 0.20 g; atrium (RA) 0.09 0.14 subjects 0.02 0.03 g). animals, there increase myocyte nuclear volume (97 μm 3 –183.6 ) (34.2 –57.2 ), which more intense shorter time, fibrosis (5.9%–10.4% vs. 0.96%–1.18%) longer. echocardiography result, myocardial thickness groups (0.09–0.11 cm subjects–0.04–0.05 cm), but variation diastolic diameter. From 2 weeks PAB, S-wave (S’) (0.031 cm/s 0.040 cm/s), fractional area change (FAC) (51%–56%), systolic function lower than those respective (0.040 0.050 61%–67%). Furthermore, genes related hypertrophy extracellular matrix initial apoptosis longer observed RV. On other hand, different between without PAB. greater (74 –136 ; 40.8 –46.9 4- 8-week (1.2% 2.2%) (0.4% 0.7%). Echocardiography showed diameter subjects. Measurements isovolumetric relaxation E-wave deceleration at subgroups, study. increased various functions, particularly hypertrophy. Conclusion: 1) Rats compatible Such alterations mediated alterations, coincide onset fibrosis. 2) Structural RV, such weight, thickness, volume, degree fibrosis, modified exposure variations highlighting an alpha beta pattern early late 3) suggests left ventricle developed histological accompanied modifications simultaneously found ventricle.