Comparative transcript profiling by SuperSAGE identifies novel candidate genes for controlling potato quantitative resistance to late blight not compromised by late maturity

• DOI: 10.3389/fpls.2013.00423
• Publication Date: 2013-11-14T07:56:02Z
• Authors: Astrid M. Draffehn; Li Li; Nicolas Krezdorn; Jia Ding; Jens Lübeck; Josef Strahwald; Meki S. Muktar; Birgit Walkemeier; Björn Rotter; Christiane Gebhardt
• Keywords: Phytophthora infestans; Candidate gene; Oomycete; Molecular breeding

Resistance to pathogens is essential for survival of wild and cultivated plants. Pathogen susceptibility causes major losses crop yield quality. Durable field resistance combined with high other superior agronomic characters are therefore, important objectives in every breeding program. Precision efficacy can be enhanced by molecular diagnostic tools, which result from knowledge the basis susceptibility. Breeding uses conferred single R genes polygenic quantitative resistance. The latter partial but considered more durable. Molecular mechanisms plant pathogen interactions elucidated mainly experimental systems involving genes, whereas most crops like potato unknown. Quantitative Phytophthora infestans causing late blight often compromised maturity, a negative character. Our objective was identify candidate not maturity. We used DNA-markers select plants different levels maturity corrected (MCR) compared their leaf transcriptomes before after infection P. using SuperSAGE (serial analysis gene expression) technology next generation sequencing. identified 2034 transcripts up or down regulated upon infection, including homolog kiwi fruit allergen kiwellin. 806 showed differential expression between groups genotypes contrasting MCR levels. observed patterns suggest that part controlled transcript uninfected Functional annotation suggests that, besides biotic abiotic stress responses, general cellular processes such as photosynthesis, protein biosynthesis, degradation play role MCR.