Quantitative Disease Resistance under Elevated Temperature: Genetic Basis of New Resistance Mechanisms to Ralstonia solanacearum

• DOI: 10.3389/fpls.2017.01387
• Publication Date: 2017-08-22T03:55:00Z
• Authors: Nathalie Aoun; Laetitia Tauleigne; Fabien Lonjon; Laurent Deslandes; Fabienne Vailleau; Fabrice Roux; Richard Berthomé
• Keywords: Ralstonia solanacearum; Bacterial wilt; Strain (injury); Wilting

In the context of climate warming, plants will be facing an increased risk epidemics as well emergence new highly aggressive pathogen species. Although a permanent increase temperature strongly affects plant immunity, underlying molecular mechanisms involved are still poorly characterized. this study, we aimed to uncover genetic bases resistance remaining efficient at elevated Ralstonia solanacearum species complex (RSSC), one most harmful phytobacteria causing bacterial wilt. To start identification quantitative trait loci (QTLs) associated with natural variation response R. solanacearum, adopted genome wide association (GWA) approach using 176 worldwide accessions Arabidopsis thaliana inoculated GMI1000 strain. Following two different procedures root-inoculation (root apparatus cut vs uncut), were incubated either 27°C or 30°C, latter mimicking in temperature. At 27°C, RPS4/RRS1-R locus was main QTL detected regardless method inoculation used. This highlights power GWA mapping identify functionally important for although developed wilting symptoms, several QTLs identified depending on We focused region strain early stages infection and, by adopting reverse approach, validated involvement strictosidine synthase-like 4 (SSL4) protein that shares structural similarities animal proteins known play role immunity.