Accumulation Profiles of Embryonic Salt-Soluble Proteins in Maize Hybrids and Parental Lines Indicate Matroclinous Inheritance: A Proteomic Analysis

• DOI: 10.3389/fpls.2017.01824
• Publication Date: 2017-10-25T08:32:07Z
• Authors: Fen Ning; Xiaolin Wu; Hang Zhang; Zhaokun Wu; Liangjie Niu; Hao Yang; Wei Wang
• Keywords: Inbred strain; Glutelin

Maize is one of the most widely cultivated crops. It accumulates a large quantity seed storage proteins, which are important for development and germination, contribute to nutritional quality seeds. Based on solubility, proteins divided into albumins (water-soluble), globulins (salt-soluble), prolamins (alcohol-soluble), glutelins (acid- or alkali-soluble). hybrids due superior performance F1 than that their parents, phenomenon known as heterosis. However, accumulation patterns in maize embryos between parental inbred lines have not been compared. In present study, two elite China, Zheng 58 Chang 7-2, reciprocal (Zheng × 7-2 58) were used explore influences embryos. For this purpose, we focused salt-soluble (SSPs) our experiments. The SSPs selectively extracted from mature after extensive removal water-soluble albumin separated using two-dimensional gel electrophoresis (2-DE), followed by mass spectrometry analysis. Our results indicated 2-DE SSP profiles closely resembled those maternal parent rather paternal parent. other words, more similar whereas such although all four types quite similar. total, 12 relatively abundant spots representing five kinds identified, nine protein displayed non-additive at least hybrid. This study provided additional data dominance partial effects Besides, earlier studies globulin-1 (also vicilin), embryos, also support above results. would be helpful revealing mechanisms underlying hybrids.