The Terpene Synthase Gene Family of Carrot (Daucus carota L.): Identification of QTLs and Candidate Genes Associated with Terpenoid Volatile Compounds

• DOI: 10.3389/fpls.2017.01930
• Publication Date: 2017-11-09T04:32:50Z
• Authors: Jens Keilwagen; Heike Lehnert; Thomas Berner; Holger Budahn; Thomas Nothnagel; Detlef Ulrich; Frank Dunemann
• Keywords: Terpene; Candidate gene; Daucus carota; Chemotype

Terpenes are an important group of secondary metabolites in carrots influencing taste and flavor, some them might also play a role as bioactive substances with impact on human physiology health. Understanding the genetic molecular basis terpene synthases (TPS) involved biosynthesis volatile terpenoids will provide insights for improving breeding strategies aimed at quality traits developing specific carrot chemotypes possibly useful pharmaceutical applications. Hence, combination metabolite profiling, genotyping-by-sequencing (GBS), genome-wide association study (GWAS) was used this work to get into control identify several TPS candidate genes that be production mono- sesquiterpenes. In panel 85 cultivars accessions, profiling 31 terpenoid compounds leaves roots, GBS approach dense marker coverage (>168,000 SNPs). Based data, total 30 QTLs identified 15 volatiles. Most were detected monoterpene ocimene, sabinene, β-pinene, borneol bornyl acetate. We 27 genomic regions all 9 chromosomes by GWAS which both associated high significance (LOD ≥ 5.91) distinct sesquiterpene genes, have been homology-based gene prediction utilizing RNA-seq data. total, 65 models assigned known plant subfamilies exception TPS-d TPS-h. TPS-b largest subfamily 32 genes.