Diversity in Grain Amaranths and Relatives Distinguished by Genotyping by Sequencing (GBS)

• DOI: 10.3389/fpls.2017.01960
• Publication Date: 2017-11-16T23:19:29Z
• Authors: Xingbo Wu; Matthew W. Blair
• Keywords: Amaranthus cruentus; Germ plasm; Illumina dye sequencing

The genotyping by sequencing (GBS) method has become a molecular marker technology of choice for many crop plants because its simultaneous discovery and evaluation large number single nucleotide polymorphisms (SNPs) utility germplasm characterization. Genome representation complexity reduction are the basis GBS fingerprinting can vary species based on genome size other sequence characteristics. Grain amaranths set three that were domesticated in New World to be high protein, pseudo-cereal grain crops. goal this research was employ technique diversity amaranth accessions close relatives from six Amaranthus determine genetic differences similarities between groupings. A total 10,668 SNPs discovered 94 with ApeKI 10X coverage Illumina sequencing. majority specific 4,568 3,082 two originating Central America cruentus A. hypochondriacus 3,284 found amongst both caudatus, originally South America, relative, quitensis. distance matrix shared alleles provided information relationships cultivated American each wild other, as distinguished outgroup species, powellii retroflexus. data also admixture pair geographical origins seed colors accessions. we here used development future study.