Comparative Proteome Analysis of Wheat Flag Leaves and Developing Grains Under Water Deficit

• DOI: 10.3389/fpls.2018.00425
• Publication Date: 2018-04-10T07:03:28Z
• Authors: Xiong Deng; Yue Liu; Xuexin Xu; Dongmiao Liu; Genrui Zhu; Xing Yan; Zhimin Wang; Yueming Yan
• Keywords: Flag (linear algebra); Proteome

In this study, we performed the first comparative proteomic analysis of wheat flag leaves and developing grains in response to drought stress. Drought stress caused a significant decrease several important physiological biochemical parameters grain yield traits, particularly those related photosynthesis starch biosynthesis. contrast, some key indicators were significantly increased, including malondialdehyde, soluble sugar, proline, glycine betaine, abscisic acid content, peroxidase activity. Two-dimensional difference gel electrophoresis (2D-DIGE) identified 87 132 differentially accumulated protein (DAP) spots representing 66 105 unique proteins following exposure grains, respectively. The proteomes two organs varied markedly, most DAPS oxidative response, energy metabolism, particular, DAPs mainly participated while primarily involved carbon metabolism response. Western blotting quantitative real-time polymerase chain reaction (qRT-PCR) further validated such as rubisco large subunit (RBSCL), ADP glucose pyrophosphorylase (AGPase), chaperonin 60 alpha (CPN-60 alpha) oxalate oxidase 2 (OxO 2). potential functions revealed that complex network synergistically regulates resistance during development. Our results from proteome perspective provide new insight into molecular regulatory mechanisms used by different respond