BnaMPK3 Is a Key Regulator of Defense Responses to the Devastating Plant Pathogen Sclerotinia sclerotiorum in Oilseed Rape

• DOI: 10.3389/fpls.2019.00091
• Publication Date: 2019-02-08T04:53:16Z
• Authors: Zheng Wang; Ling-Li Bao; Feng-Yun Zhao; Min-Qiang Tang; Ting Chen; Yaoming Li; Bing-Xu Wang; Benzhong Fu; Hedi Fang; Guan-Ying Li; Jun Cao; Li-Na Ding; Ke-Ming Zhu; Sheng-Yi Liu; Xiao-Li Tan
• Keywords: Sclerotinia Sclerotiorum; Jasmonic acid

The disease caused by Sclerotinia sclerotiorum has traditionally been difficult to control, resulting in tremendous economic losses oilseed rape (Brassica napus). Identification of important genes the defense responses is critical for molecular breeding, an strategy controlling disease. Here, we report that a B. napus mitogen-activated protein kinase gene, BnaMPK3, plays role against S. rape. BnaMPK3 highly expressed stems, flowers and leaves, its product localized nucleus. Furthermore, responsive infection treatment with jasmonic acid (JA) or biosynthesis precursor ethylene (ET), but not salicylic (SA) abscisic acid. Moreover, overexpression (OE) Nicotiana benthamiana results significantly enhanced resistance sclerotiorum, whereas diminished RNAi transgenic plants. After infection, associated ET, JA SA signaling are intensified BnaMPK3-OE plants weakened BnaMPK3-RNAi when compared those wild type plants; contrast level both H2O2 accumulation cell death exhibits reverse pattern. candidate gene association analyses show BnaMPK3-encoding BnaA06g18440D locus cause variation natural population. These suggest key regulator multiple which may guide improvement related crops.