Comparative Metabolomic and Transcriptomic Studies Reveal Key Metabolism Pathways Contributing to Freezing Tolerance Under Cold Stress in Kiwifruit

• DOI: 10.3389/fpls.2021.628969
• Publication Date: 2021-06-01T18:13:58Z
• Authors: Shihang Sun; Jinbao Fang; Miaomiao Lin; Chungen Hu; Xiujuan Qi; Jinyong Chen; Yunpeng Zhong; Abid Muhammad; Zhi Li; Yukuo Li
• Keywords: Metabolome; KEGG; Metabolic pathway; Phenylpropanoid

Cold stress poses a serious treat to cultivated kiwifruit since this plant generally has weak ability tolerate freezing tolerance temperatures. Surprisingly, however, the underlying mechanism of kiwifruit’s remains largely unexplored and unknown, especially regarding key pathways involved in conferring trait. Here, we studied metabolome transcriptome profiles freezing-tolerant genotype KL ( Actinidia arguta ) freezing-sensitive RB A. ), identify main important metabolites related their tolerance. A total 565 were detected by wide-targeting metabolomics method. Under (−25°C) cold stress, KEGG (Kyoto Encyclopedia Genes Genomes) pathway annotations showed that flavonoid metabolic specifically upregulated KL, which increased its scavenge for reactive oxygen species (ROS). The changes identified accompanied specific upregulation codeinone reductase gene, chalcone isomerase an anthocyanin 5-aromatic acyltransferase gene. Nucleotides metabolism phenolic acids RB, indicated had higher energy weaker dormancy ability. Since LPCs (LysoPC), LPEs (LysoPE) free fatty accumulated simultaneously both genotypes, these could serve as biomarkers cold-induced frost damages. These components evidently participated regulation genotypes. In conclusion, results study demonstrated inherent differences composition activity between under conditions.