Direct and Indirect Somatic Embryogenesis Induction in Camellia oleifera Abel

• DOI: 10.3389/fpls.2021.644389
• Publication Date: 2021-03-26T14:53:30Z
• Authors: Ming Zhang; Aibin Wang; Mou Qin; Xuejing Qin; Shiwen Yang; Shuchai Su; Yongjiang Sun; Lingyun Zhang
• Keywords: Camellia oleifera; Callus

Camellia oleifera Abel. is an important woody oil species; however, the shortage of rapid and industrialized seedling culture a large constraint on development tea industry. Somatic embryogenesis (SE) one main powerful biotechnological tools for plant mass regeneration, but largely unknown SE in C. limits scale production clonal plants. In this study, we described high-efficiency system via direct indirect pathways investigated effect genotype, explant age phytohormones SE. pathway, somatic embryos were highly induced from immature seeds 220 days after full blossom, embryoids was achieved with combination 0.19 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) 0.05 thidiazuron (TDZ). embryogenic calli same explants medium containing 1.5 2,4-D, while 0.75 2,4-D treatment led to high proliferation rates calli. The addition alone stimulated globular causing 75% loss induction rate heart embryo stage. Upon transfer phytohormone-free medium, optimal 62.37% cotyledonary obtained. These data suggest that subsequent differentiation process stage ISE more similar endogenous phytohormones-driven process. Mature germinated produce intact plantlets half-strength MS basal regeneration 63.67%. Histological analysis confirmed vascular bundle isolation mother tissue. We further studied different varieties found there no significant genotype differences efficiency . Thus, established (ISE) regenerated SE, not organogenesis. has complicated regulatory mechanism than embryogenesis. improved protocol would benefit propagation genetic manipulation