Screening of Key Proteins Affecting Floral Initiation of Saffron Under Cold Stress Using iTRAQ-Based Proteomics

• DOI: 10.3389/fpls.2021.644934
• Publication Date: 2021-05-11T06:33:28Z
• Authors: Jing Chen; Guifen Zhou; Yan Dong; Xiaodong Qian; Jing Li; Xuting Xu; Huilian Huang; Limin Xu; Liqin Li
• Keywords: Sucrose-phosphate synthase; Metabolic pathway

Saffron crocus (Crocus sativus) is an expensive and valuable species that presents preventive curative effects. This study aimed to screen the key proteins affecting floral initiation of saffron under cold stress thus increasing yield by regulating temperature.Protein expression profiles in flowering non-flowering buds were established using isobaric tags for relative or absolute quantitation (iTRAQ). A total 5,624 identified, 201 differentially abundant protein (DAPs) further obtained between groups. The most important functions upregulated DAPs "sucrose metabolic process," "lipid transport," "glutathione "gene silencing RNA." Downregulated significantly enriched "starch biosynthetic process" several oxidative response pathways. Three new flower-related proteins, CsFLK, CseIF4a, CsHUA1, identified this study. following eight genes validated real-time qPCR top from five different growth phases: induction- organ development-related CseIF4A, CsGSTU7; sucrose synthase activity-related CsSUS1 CsSUS2; starch CsGBSS1 CsPU1. These findings demonstrate roles played sucrose/starch biosynthesis pathways development at mRNA level. During normal development, contents increased, decreased. In contrast, showed decreased no significant changes compared with those dormancy stage.In report, a environment revealed first time iTRAQ. possible "reactive oxygen species-antioxidant system-starch/sugar interconversion pathway" was explain phenomenon does not bloom due low temperature treatment.