Genotype-Specific Expression and NLR Repertoire Contribute to Phenotypic Resistance Diversity in Plantago lanceolata

• DOI: 10.3389/fpls.2021.675760
• Publication Date: 2021-07-12T05:44:52Z
• Authors: Pezhman Safdari; Layla Höckerstedt; Mikael Brosche; Jarkko Salojärvi; Anna-Liisa Laine
• Keywords: 0303 health sciences; Phenotypic Variation; pathogen-imposed selection; :Biological sciences [Science]; Plant culture; phenotypic variation; Plant Science; 11831 Plant biology; SB1-1110; expression profile; 03 medical and health sciences; Ecology, evolutionary biology; phenotypic resistance diversity; natural host populations; Pathogen-Imposed Selection

High levels of phenotypic variation in resistance appears to be nearly ubiquitous across natural host populations. Molecular processes contributing this nature are still poorly known, although theory predicts evolve at specific loci driven by pathogen-imposed selection. Nucleotide-binding leucine-rich repeat (NLR) genes play an important role pathogen recognition, downstream defense responses and signaling. Identifying the NLRs has potential increase our understanding how NLR diversity is generated maintained, manage disease resistance. Here, we sequenced transcriptomes five different Plantago lanceolata genotypes when inoculated same strain obligate fungal Podosphaera plantaginis . A de novo transcriptome assembly RNA-sequencing data yielded 24,332 gene models with N50 value 1,329 base pairs space completeness 66.5%. The expression showed highly varying where each plant genotype demonstrated a unique profile response pathogen, regardless phenotype. Analysis on conserved NB-ARC domain diverse repertoire P. consistent high species. We find evidence selection generating some loci. Jointly, results demonstrate that from crosstalk between mechanisms. In conclusion, characterizing architecture populations may shed unprecedented light evolution generate variation.