Study of Terpenoid Synthesis and Prenyltransferase in Roots of Rehmannia glutinosa Based on iTRAQ Quantitative Proteomics

• DOI: 10.3389/fpls.2021.693758
• Publication Date: 2021-08-04T07:46:34Z
• Authors: Peilei Chen; Xiaoyan Wei; Qianting Qi; Wenjing Jia; Mingwei Zhao; Huina Wang; Yanqing Zhou; Hongying Duan
• Keywords: Rehmannia glutinosa; KEGG; Metabolic pathway

Rehmannia glutinosa has important medicinal value; terpenoid is one of the main active components in R. . In this study, iTRAQ technique was used to analyze relative abundance proteins roots , and 6,752 reliable were quantified. GO enrichment results indicated that most involved metabolic process or cellular process, 57.63% had catalytic activity, 65.80% enriched membrane-bounded organelle. there 38 KEGG enrichments with significance, more DEPs found some pathways, especially proteasome pathway TCA cycle 15.0% between elongation stage expansion roots. Furthermore, five pathways synthesis found. Most prenyltransferases belong FPP/GGPP synthase family, backbone biosynthesis, all interacted biotin carboxylase CAC2. Compared at stage, many exhibited higher expression maturation addition, eight encoding genes cloned from namely FPPS, FPPS1, GGPS, GGPS3, GGPS4, GGPS5, GPPS GPPS2 introns also GGPS5 GGPS2 FPP/GPP synthases conservative organisms, viridiplantae, which co-occurrence significantly plants. Further analysis divided into three kinds, their gene diverse different varieties, growth periods, tissues GGPS FPPS much stage. Thus, terpenoids intricately regulated needs be further studied.