A Comparison of Two Monoterpenoid Synthases Reveals Molecular Mechanisms Associated With the Difference of Bioactive Monoterpenoids Between Amomum villosum and Amomum longiligulare

• DOI: 10.3389/fpls.2021.695551
• Publication Date: 2021-08-12T10:23:21Z
• Authors: Haiying Zhao; Meng Li; Yuanyuan Zhao; Xiaojing Lin; Huilin Liang; Jieshu Wei; Wuke Wei; Dongming Ma; Zhongyu Zhou; Jinfen Yang
• Keywords: Borneol; Camphene; Terpene

The fruits of Amomum villosum and longiligulare are both used medicinally as Fructus Amomi the famous traditional Chinese medicine, however, medicinal quality A. is better than that . Volatile terpenoids in seeds, especially bornyl acetate borneol, components Amomi. volatile transcriptome developing seeds were compared this study. result revealed borneol contents higher Additionally, six terpenoid synthase genes ( AlTPS1–AlTPS6 ) screened from , AlTPS2 AlTPS3 found to share 98 83% identity with AvTPS2 AvBPPS (bornyl diphosphate synthase) respectively. BPPS key enzyme for biosynthesis acetate. Biochemical assays improved had an identical function linalool synthase; produced camphene major product (BPP) secondary product, whereas BPP its product. There was only one different amino acid between (A496) (G495) their conserved motifs, site-directed mutation A496G DTE motif changed BPP. Molecular docking suggests narrows camphene-binding pocket decreases BPP-binding energy, thus increases selectivity enzyme. In addition, expression level significantly which consistent related-metabolites contents. This study provides insight into TPS-related molecular bases accumulation differences bioactive gene involved active compound, identified a target could be applied quality-related identification breeding