Genome-Wide Analysis of CCT Transcript Factors to Identify Genes Contributing to Photoperiodic Flowering in Oryza rufipogon

• DOI: 10.3389/fpls.2021.736419
• Publication Date: 2021-11-08T07:18:41Z
• Authors: Xin Peng; Win Tun; Shuang-feng Dai; Jia-yue Li; Qun-jie Zhang; Guo-ying Yin; Jinmi Yoon; Lae-hyeon Cho; Gynheung An; Li-zhi Gao
• Keywords: Oryza rufipogon; Oryza

Photoperiod sensitivity is a dominant determinant for the phase transition in cereal crops. CCT (CONSTANS, CO-like, and TOC1) transcription factors (TFs) are involved many physiological functions including regulation of photoperiodic flowering. However, functional roles TFs have not been elucidated wild progenitors In this study, we identified 41 TFs, 19 CMF, 17 COL, five PRR Oryza rufipogon, presumed ancestor Asian cultivated rice. There thirty-eight orthologous genes sativa, which ten pairs duplicated shared with O. rufipogon. We investigated daily expression patterns, showing that 36 OrCCT exhibited circadian rhythmic expression. A total thirteen were as putative flowering suppressors rufipogon based on developmental patterns transgenic phenotypes. propose OrCCT08, OrCCT24, OrCCT26 strong alleles rice DTH2, Ghd7, OsPRR37, respectively. The SD treatment at 80 DAG stimulated LD-grown plants. Our results further showed nine significantly downregulated under treatment. findings would provide valuable information construction regulatory network characterization both sativa.