Identification of the Complex Interplay Between Nematode-Related lncRNAs and Their Target Genes in Glycine max L.

• DOI: 10.3389/fpls.2021.779597
• Publication Date: 2021-12-10T14:48:10Z
• Authors: Masoumeh Ahmadi K...; Marzieh Karimi; Roya Karamian; Sahand Amini; Aboozar Soorni
• Keywords: Soybean cyst nematode

Soybean (Glycine max) is a major plant protein source and oilseed crop. However, plant-parasitic nematodes (PPNs) affect its annual yield. In the current study, in order to better understand regulation of defense mechanism against PPNs soybean, we investigated role long non-coding RNAs (lncRNAs) response two nematode species, Heterodera glycines (SCN: soybean cyst nematode) Rotylenchulus reniformis (reniform). To this end, publicly available RNA-seq data sets (SCN set RAD: reniform-associated set) were employed discover lncRNAome profile under SCN reniform infection, respectively. Upon identification unannotated transcripts these sets, seven-step pipeline was utilized sieve transcripts, which ended up 384 283 potential lncRNAs RAD, These then used predict cis trans nematode-related targets genome. Computational prediction target genes function, some also among differentially expressed genes, revealed involvement putative nematode-responsive as well enrichment multiple stress responses both sets. Finally, 15 six proposed be involved microRNA-mediated gene expression SNC Collectively, study provides novel insight into signaling regulatory network soybean-pathogen interactions opens new window for further research.