Genome-Wide Identification of JRL Genes in Moso Bamboo and Their Expression Profiles in Response to Multiple Hormones and Abiotic Stresses

• DOI: 10.3389/fpls.2021.809666
• Publication Date: 2022-01-14T04:40:42Z
• Authors: Zhijun Zhang; Bin Huang; Jialu Chen; Yang Jiao; Hui Guo; Shenkui Liu; Muthusamy Ramakri...; Guoning Qi
• Keywords: Phyllostachys edulis; Subfamily; Methyl jasmonate; Brachypodium

Jacalin-related lectins (JRLs) are a new subfamily of plant that has recently been recognized and plays an important role in growth, development, abiotic stress response. Although moso bamboo (Phyllostachys edulis) is economically industrially worldwide, there no systematic identification JRLs this species. Here, we identified 25 JRL genes bamboo, these unequally distributed among 10 genome scaffolds. Phylogenetic analysis showed the were clustered into four subgroups: I, II, V, VII. Numerous stress-responsive hormone-regulated cis-elements detected upstream promoter regions JRLs. Genome collinearity analyses more closely related to those Brachypodium distachyon than Oryza sativa Zea mays. Sixty-four percent PeJRL present as segmental tandem duplicates. qRT-PCR expression same subgroup significantly downregulated response salicylic acid (SA), abscisic (ABA), methyl jasmonate (MeJA) treatments upregulated under low temperature, drought, salt stress; they also exhibited tissue-specific patterns. Subcellular localization experiments revealed PeJRL04 PeJRL13 localized cell membrane, nucleus, cytoplasm. Three dimensional structure prediction yeast two-hybrid assays used verify exists self-interacting homodimer vivo. These findings provide reference for understanding functions specific effective selection stress-related genes.