On the nature of the plant ER exit sites

• DOI: 10.3389/fpls.2022.1010569
• Publication Date: 2022-10-07T11:57:16Z
• Authors: Alastair J. McGin...; Jennifer Schoberer; Charlotte Pain; Federica Brandizzi; Verena Kriechbaumer
• Keywords: COPII; COPI; Organelle; Brefeldin A

In plants, the endoplasmic reticulum (ER) and Golgi bodies are not only in close proximity, but also physically linked. This unique organization raises questions about nature of transport vectors carrying cargo between two organelles. Same as metazoan yeast cells, it was suggested that is transported from ER to cisternae via COPII-coated vesicles produced at ribosome-free exit sites (ERES). Recent developments mammalian cell research suggest, though, COPII helps select secretory cargo, does coat carriers leaving ER. Furthermore, shown ERES expand into a tubular network containing no components. Because association plant previously proposed comprise unit travels over or with motile membrane. this study, we aimed explore cells took advantage high-resolution confocal microscopy imaged labelled canonical markers (Sar1a, Sec16, Sec24). We found dynamically connected most likely represent pre- cis -Golgi cisternae. showed fine connections compartments (ERGo tubules) well protrusions ERES/Golgi connecting suggest these tubules observed involved stabilizing physical connection cisternae, may be bodies.