Transcriptome analysis reveals the proline metabolic pathway and its potential regulation TF-hub genes in salt-stressed potato

• DOI: 10.3389/fpls.2022.1030138
• Publication Date: 2022-10-17T07:45:43Z
• Authors: Quankai Jing; Hualan Hou; Xiaoke Meng; Airu Chen; Lixia Wang; Husen Zhu; Shuang Zheng; Zhaoyan Lv; Xiaobiao Zhu
• Keywords: Metabolic pathway; KEGG

Potato (Solanum tuberosum) is currently the third most important food crop in world. However, production of potato seriously threatened by salt stress, which often occurs facility cultivation environment, and mining tolerance genes remains to be further studied. In this study, test-tube plantlets DM were treated with 200-mM NaCl simulate 15 cDNA libraries constructed for RNA-seq analysis. A total 8383 DEGs identified, 3961 shared among all treatments, 264 (7.15%) TF-coding identified from these DEGs. KEGG enrichment analysis showed that "arginine proline metabolism" (ko00330) enriched metabolic pathway, their functions almost covered whole process. Further expression levels 13 structural pathway significantly up-regulated accumulation was also increased under TF-hub discovered WGCNA lightcyan tan modules highly positively correlated contents. Correlation revealed four module seven might potential candidate genes, especially novel regulatory gene StGLK014720. Furthermore, dual-luciferase reporter assay confirmed key protein StGLK014720 could activate promoters both StAST021010 StAST017480. conclusion, results lay foundation study on mechanism potato, provide a theoretical basis new genetic resources breeding potato.