Genome-wide analysis of the Populus trichocarpa laccase gene family and functional identification of PtrLAC23

• DOI: 10.3389/fpls.2022.1063813
• Publication Date: 2023-01-17T08:14:47Z
• Authors: Boyang Liao; Chencan Wang; Xiaoxu Li; Yi Man; Hang Ruan; Yuanyuan Zhao
• Keywords: Populus trichocarpa

Introduction Biofuel is a kind of sustainable, renewable and environment friendly energy. Lignocellulose from the stems woody plants main raw material for “second generation biofuels”. Lignin content limits fermentation yield therefore major obstacle in biofuel production. Plant laccase plays an important role final step lignin formation, which provides new strategy us to obtain ideal biofuels by regulating expression genes directly gain desired or change composition lignin. Methods Multiple sequence alignment phylogenetic analysis were used classify PtrLAC genes; features PtrLACs revealed gene structure motif analysis; duplication, interspecific collinearity Ka/Ks conducted identify ancient ; levels measured RNA-Seq data qRT-PCR; domain combine with cis-acting elements prediction together showed potential function . Furthermore, Alphafold2 was simulate 3D structures, proLAC23::LAC23-eGFP transgenic Populus stem transects applied fluorescence observation. Results A comprehensive P. trichocarpa ( PtLAC ) family performed. Some such as PtrLAC25 , PtrLAC19 PtrLAC41 identified. Gene distribution conserved motifs clearly characteristics each Combining published qRT-PCR analysis, we pattern family. Prediction results show that regulation closely related light. Through above analyses, selected 5 laccases protein PtrLAC23 may be lignification. Fluorescence observation confirmed our hypothesis again. Discussion In this study, fully analyzed identified key These findings not only provide insights into functions laccase, but also give understanding broad prospects plant lignocellulosic