GhCDPK60 positively regulates drought stress tolerance in both transgenic Arabidopsis and cotton by regulating proline content and ROS level

• DOI: 10.3389/fpls.2022.1072584
• Publication Date: 2022-12-01T17:57:24Z
• Authors: Mengyuan Yan; Xiaotian Yu; Gen Zhou; Dongli Sun; Yu Hu; Chenjue Huang; Qintao Zheng; Nan Sun; Jiayan Wu; Zhaobin Fu; Libei Li; Zhen Feng; Shuxun Yu
• Keywords: Drought Tolerance; Gossypium; Osmotic shock

Calcium-Dependent Protein Kinases (CDPKs) involved in regulating downstream components of calcium signaling pathways play a role tolerance to abiotic stresses and seed development plants. However, functions only few cotton CDPKs have been clarified at present. In this study, 80 conserved Gossypium hirsutum L. were identified characterized, which was divided into four subgroups. Among them, the transcript level GhCDPK60 significantly upregulated under drought several hormone treatments. And we found that expression levels stress-inducible genes down-regulated -silence up-regulated -overexpressing Arabidopsis . addition, physiological analyses demonstrated improved stress by improving osmotic adjustment ability reducing accumulation reactive oxygen species (ROS) These findings broaden our understanding biological roles mechanisms underlying cotton.