Biocontrol of the causal brown patch pathogen Rhizoctonia solani by Bacillus velezensis GH1-13 and development of a bacterial strain specific detection method

• DOI: 10.3389/fpls.2022.1091030
• Publication Date: 2023-01-10T12:38:36Z
• Authors: Gahee Lee; Hyeongju Choi; Haifeng Liu; Yun-Hyeong Han; Narayan Chandra Paul; Gui Hwan Han; Hyunsook Kim; Pyoung Il Kim; Sun-Il Seo; Jaekyeong Song; Hyunkyu Sang
• Keywords: Rhizoctonia

Brown patch caused by the basidiomycete fungus Rhizoctonia solani is an economically important disease of cool-season turfgrasses. In order to manage disease, different types fungicides have been applied, but negative impact on environment continues rise. this study, beneficial bacteria Bacillus velezensis GH1-13 was characterized as a potential biocontrol agent brown disease. The strain strongly inhibited mycelial growth turf pathogens including anastomosis groups R. causing and large patch. AG2-2(IIIB) hyphae were morphologically changed, fungal cell death resulted from exposure GH1-13. addition, compatibility with bacterial strain, combined application fungicide azoxystrobin in control creeping bentgrass indicated that could serve agent. To develop strain-specific detection method, two unique genes chromosome plasmid found using pan-genome analysis 364 strains. gene successfully detected both SYBR Green TaqMan qPCR methods DNA or soil samples. This study suggests offers environmentally friendly approach via reducing rates. Furthermore, developed pipeline method be useful tool for detecting studying dynamics specific agents.