Modulation in Biofertilization and Biofortification of Wheat Crop by Inoculation of Zinc-Solubilizing Rhizobacteria

• DOI: 10.3389/fpls.2022.777771
• Publication Date: 2022-02-25T09:34:57Z
• Authors: Ramesh Chandra Yadav; Sushil K. Sharma; Ajit Varma; Mahendra Vikram S...; Mohammad Shavez Khan; Pawan K. Sharma; Deepti Malviya; Udai B. Singh; Jai P. Rai; Anil K. Saxena
• Keywords: Biofortification

Zinc is an important micronutrient needed for the optimum growth and development of plants. Contrary to chemical zinc fertilizers, use zinc-solubilizing bacteria environmentally friendly option enrichment in edible parts crops. This study was conducted with objective selecting potential rhizobacteria from rhizosphere chickpea grown soils eastern Uttar Pradesh further assessing their impact on magnitude assimilation wheat Among 15 isolates, CRS-9, CRS-17, CRS-30, CRS-38 produced net soluble broth tune 6.1, 5.9, 5.63, 5.6 μg ml-1, respectively, phosphate corresponding pH 4.48, 5.31, 5.2, 4.76. However, bacterial strains CRS-38, CRS-9 showed maximum solubilization efficiency 427.79, 317.39, 253.57, 237.04%, respectively. The four isolates were identified as Bacillus glycinifermentans Microbacterium oxydans Paenarthrobacter nicotinovorans tequilensis basis morphological biochemical studies 16S rRNA gene sequencing. Bacterial inoculants significantly colonized roots plants formed a biofilm root matrix. These increased seed germination (%) vigor indices under glasshouse conditions. After 30 days sowing microcosm conditions, eight transporter (TaZIP) genes expressed maximally roots, concomitant accumulation higher content bacterially treated plant compared absolute control. Out tested, two bacteria, B. P. improved (%), (2-2.5 folds), biomass, grain yield (2.39 g plant-1), biofortificated grains (54.25 g-1Zn) wheat. To best our knowledge, this may be first report presence trait M. CRS-30.