Construction of a High-Density Genetic Map and Identification of Quantitative Trait Loci Linked to Fruit Quality Traits in Apricots Using Specific-Locus Amplified Fragment Sequencing

Prunus armeniaca Genetic linkage Candidate gene
DOI: 10.3389/fpls.2022.798700 Publication Date: 2022-02-14T05:39:02Z
ABSTRACT
Improving fruit quality is one of the main tasks in modern commercial apricot breeding. Because lack high-density linkage maps and fine mapping, it difficult to obtain molecular markers that can assist breeding for quantitative inheritance traits. In this study, specific-locus amplified fragment sequencing was used genotype 169 seedlings F1 (Prunus armeniaca L.) progenies derived from crossing "Chuanzhihong" (H) with "Saimaiti" (S). After aligning Prunus reference genome filtering out low-quality variants, 6,012 high-quality single nucleotide polymorphisms were obtained employed construct a genetic map each parent. The showed eight groups apricot, covering distance 809.6 cM "H" 1076.4 "S". average between "S" 0.62 0.95 cM, respectively. To trait loci (QTLs) quality, we investigated traits, including weight (FW), height (FH), lateral width (FL), ventral (FV), soluble solids content (SSC), firmness (FF) all genotyped 2018 2019. Eleven nine QTLs linked traits anchored on maps, respectively, 1,138 putative candidate genes 16 most significant regions corresponding chromosome identified based gene annotation. Among them, size contained 648 11 intervals genome, SSC 372 3 intervals, FF 117 2 intervals. Our findings uncovered basis provided further studies QTL targets future marker-assisted selection improvement
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