Identification of Arabidopsis Phospholipase A Mutants With Increased Susceptibility to Plasmodiophora brassicae

• DOI: 10.3389/fpls.2022.799142
• Publication Date: 2022-02-18T09:25:40Z
• Authors: Qinqin Zhou; Kethmi N. Jayawar...; Stephen E. Strelkov; Sheau-Fang Hwang; Guanqun Chen
• Keywords: Clubroot; Brassicaceae

Clubroot, caused by the obligate parasite Plasmodiophora brassicae, is one of most devastating diseases canola (Brassica napus) in Canada. The identification novel genes that contribute to clubroot resistance important for sustainable management clubroot, as these may be used development resistant cultivars. Phospholipase As (PLAs) play roles plant defense signaling and stress tolerance, thus are attractive targets crop breeding. However, since an allopolyploid has multiple copies each PLA gene, it time-consuming test functions PLAs directly this crop. In contrast, model Arabidopsis thaliana a simpler genetic background only copy PLA. Therefore, would reasonable faster validate potential utility first. study, we identified seven homozygous atpla knockout/knockdown mutants Arabidopsis, tested their performance following inoculation with P. brassicae. Four (pla1-iiα, pla1-iγ3, pla1-iii, ppla-iiiβ, ppla-iiiδ) developed more severe than wild-type, suggesting increased susceptibility homologs (AtPLAs) B. napus (BnPLAs) were through Blast searches phylogenic analysis. Expression BnPLAs was subsequently examined transcriptomic datasets generated from infected promising candidates further characterization identified.