Identification of Key Gene Networks and Deciphering Transcriptional Regulators Associated With Peanut Embryo Abortion Mediated by Calcium Deficiency

• DOI: 10.3389/fpls.2022.814015
• Publication Date: 2022-03-23T00:31:35Z
• Authors: Hua Chen; Qiang Yang; Huiwen Fu; Kun Chen; Shanshan Zhao; Chong Zhang; Tiecheng Cai; Lihui Wang; Wenzhi Lu; Hao Dang; Meijia Gao; Huaqi Li; Xinyi Yuan; Rajeev K. Varshney; Weijian Zhuang
• Keywords: Identification; Gene regulatory network

Peanut embryo development is easily affected by a variety of nutrient elements in the soil, especially calcium level. produces abortive embryos calcium-deficient but underlying mechanism remains unclear. Thus, identifying key transcriptional regulators and their associated regulatory networks promises to contribute better understanding this process. In study, cellular biology gene expression analyses were performed investigate peanut with aim discern global architecture abortion under deficiency conditions. The endomembrane systems tended disintegrate, impairing cell growth starch, protein lipid body accumulation, resulting aborted seeds. RNA-seq analysis showed that profile was significantly changed deficiency. Further indicated multiple signal pathways involved abortion. Differential expressed genes (DEGs) related cytoplasmic free Ca 2+ altered. DEGs plant hormone signaling be increased IAA ethylene decreased ABA, gibberellin, cytokinin, brassinosteroid levels. Certain vital genes, including apoptosis-inducing factor, WRKYs ethylene-responsive transcription factors, up-regulated, while development, such as TCP4 , WRI1 FUS3 ABI3 GLK1 down-regulated. Weighted co-expression network (WGCNA) identified 16 significant modules signaling, MAPK ubiquitin mediated proteolysis, reserve substance biosynthesis metabolism decipher network. most module darkolivegreen2 (AH06G23930) had highest connectivity among module. Importantly, factors embryogenesis or ovule bHLH115 MYC2 etc., also present down regulated This study presents first view conditions lays foundation for improving tolerances targeted manipulation molecular breeding.