High Resolution Genome Wide Association Studies Reveal Rich Genetic Architectures of Grain Zinc and Iron in Common Wheat (Triticum aestivum L.)

• DOI: 10.3389/fpls.2022.840614
• Publication Date: 2022-03-16T08:04:56Z
• Authors: Jingyang Tong; Cong Zhao; Mengjing Sun; Luping Fu; Jie Song; Dan Liu; Yelun Zhang; Jianmin Zheng; Zongjun Pu; Lianzheng Liu; Awais Rasheed; Ming Li; Xianchun Xia; Zhonghu He; Yuanfeng Hao
• Keywords: Common wheat; Biofortification; Genome-wide Association Study

Biofortification is a sustainable strategy to alleviate micronutrient deficiency in humans. It necessary improve grain zinc (GZnC) and iron concentrations (GFeC) wheat based on genetic knowledge. However, the precise dissection of architecture underlying GZnC GFeC remains challenging. In this study, high-resolution genome-wide association studies were conducted for by three different models using 166 cultivars 373,106 polymorphic markers from 660K 90K single nucleotide polymorphism (SNP) arrays. Totally, 25 16 stable loci detected GFeC, respectively. Among them, 17 8 are likely be new quantitative trait locus/loci (QTL). Based gene annotations expression profiles, 28 promising candidate genes identified Zn/Fe uptake (8), transport (11), storage (3), regulations (6). Of 11 putative orthologs known Arabidopsis rice related homeostasis. A brief model, such as homeostasis root uptake, xylem final seed was proposed wheat. Kompetitive allele-specific PCR (KASP) successfully developed two major QTL chromosome arms 3AL 7AL, respectively, which independent thousand kernel weight plant height. The further validated bi-parental population under multi-environments. multidrug toxic compound extrusion (MATE) transporter TraesCS3A01G499300, ortholog OsPEZ2, potential gene. This study has advanced our knowledge basis provides valuable biofortification.