Plant Hormone Response to Low-Temperature Stress in Cold-Tolerant and Cold-Sensitive Varieties of Zanthoxylum bungeanum Maxim

• DOI: 10.3389/fpls.2022.847202
• Publication Date: 2022-04-29T12:54:39Z
• Authors: Jieyun Tian; Yao Ma; Yabing Chen; Xue Chen; Anzhi Wei
• Keywords: 0301 basic medicine; 2. Zero hunger; 03 medical and health sciences; WGCNA; cold stress; chemometric analysis; Plant culture; Zanthoxylum bungeanum; Plant Science; plant hormone; SB1-1110

Plant growth and survival in nature, its growth process, will be affected by various factors from the environment, among which temperature has a greater impact. In recent years, extreme weather has frequently appeared, and the growth of crops has been increasingly affected by the environment. As an important flavoring and Chinese herbal medicine crop,Zanthoxylum bungeanumis also facing the harm of low-temperature stress. Plant hormones play a vital role in the response of plants to low temperatures. In this study, ultra-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to determine the hormone components of cold-tolerant and cold-sensitive varieties ofZ. bungeanum. Combined with chemometric analysis and weighted gene co-expression network analysis (WGCNA), the hormone component differences and hormone response strategies ofZ. bungeanumunder low-temperature stress were comprehensively studied. The results showed that 45 hormones were detected inZ. bungeanum. Among them, there were 7 kinds of components with high content and were detected in both two varieties. At the late stage of low-temperature stress, the contents of abscisic acid (ABA) and ABA-glucosyl ester (ABA-GE) in Fuguhuajiao (FG) were significantly increased, and the latter served as the storage of the former to supplement the active ABA. Orthogonal partial least squares discriminant analysis (OPLS-DA) found that indole-3-carboxylic acid (ICA), indole-3-carboxaldehyde (ICAld), meta-Topolin riboside (mTR), cis-Zeatin-O-glucoside riboside (cZROG), and N6-isopentenyladenosine (IPR) in FG were the upregulated important difference components, and IPR and 2-methylthio-cis-zeatin riboside (2MeScZR) in Fengxiandahongpao (FX) were the upregulated important difference components. There were common crossing points and independent response pathways in response to low temperature in two varieties. WGCNA analysis found that the main hormone components were associated with multiple metabolic pathways including carbon, fatty acid, amino acid, and sugar metabolism, indicating that hormone regulation plays an important role in the response ofZ. bungeanumto low temperature. This study clarified the hormone response mechanism ofZ. bungeanumunder low-temperature stress and provided a reference and basis for further improving the cold resistance ofZ. bungeanumand cultivating new varieties.