MAG2 and MAL Regulate Vesicle Trafficking and Auxin Homeostasis With Functional Redundancy

• DOI: 10.3389/fpls.2022.849532
• Publication Date: 2022-03-16T07:59:36Z
• Authors: Xiaohui Ma; Xiaonan Zhao; Hailong Zhang; Yiming Zhang; Shanwen Sun; Ying Li; Zhengbiao Long; Yuqi Liu; Xiaomeng Zhang; Rongxia Li; Li Tan; Lixi Jiang; Jian-Kang Zhu; Lixin Li
• Keywords: MAG2 and MAL; 0303 health sciences; 03 medical and health sciences; vesicle trafficking; auxin homeostasis; plant development and stress response; Plant culture; Plant Science; proteomic analysis; SB1-1110

Auxin is a central phytohormone and controls almost all aspects of plant development stress response. homeostasis coordinately regulated by biosynthesis, catabolism, transport, conjugation, deposition. Endoplasmic reticulum (ER)-localized MAIGO2 (MAG2) complex mediates tethering arriving vesicles to the ER membrane, it crucial for export trafficking. Despite important regulatory roles MAG2 in vesicle trafficking, mag2 mutant had mild developmental abnormalities. has one homolog protein, MAG2-Like (MAL), mal-1 also slight phenotypes. In order investigate MAL function development, we generated mag2-1 double mutant. As expected, exhibited serious defects more alteration response compared with single mutants wild type. Proteomic analysis revealed that signaling, metabolism, were affected, especially membrane trafficking auxin transport. Biochemical cell biological indicated transport than mutants. The distribution abundance transporters altered significantly Our findings suggest regulate controlling functional redundancy.