Transfer of the ph1b Deletion Chromosome 5B From Chinese Spring Wheat Into a Winter Wheat Line and Induction of Chromosome Rearrangements in Wheat-Aegilops biuncialis Hybrids

• DOI: 10.3389/fpls.2022.875676
• Publication Date: 2022-06-13T05:55:56Z
• Authors: Edina Türkösi; László Ivanizs; András Farkas; Eszter Gaál; Klaudia Kruppa; Péter Kovács; Éva Szakács; Kitti Szőke-Pázsi; Mahmoud Said; Petr Cápal; Simon Griffiths; Jaroslav Doležel; István Molnár
• Keywords: Introgression; Aegilops; Common wheat

Effective utilization of genetic diversity in wild relatives to improve wheat requires recombination between and alien chromosomes. However, this is suppressed by the Pairing homoeologous gene, Ph1, on long arm chromosome 5B. A deletion mutant Ph1 locus (ph1b) has been used widely induce × hybrids. original ph1b mutation, developed Chinese Spring (CS) background poor agronomic performance. Hence, introgression lines are first backcrossed with adapted genotypes after step, segments introduced into breeding lines. In work, mutation was transferred from two CSph1b mutants winter line Mv9kr1. Homozygous Mv9kr1 ph1b/ph1b exhibited improved plant spike morphology compared Spring. Flow cytometric analysis confirmed reduced DNA content 5B both genotype relative type chromosome. The allowed wheat-alien pairing meiosis Mv9kr1ph1b_K Aegilops biuncialis F1 hybrids, predominantly Mb-genome chromosomes those Ub genome. High frequency wheat-Aegilops interactions resulted rearranged identified new Mv9kr1ph1b Ae. Biuncialis amphiploids, making these valuable sources for introgressions. a unique resource support hexaploid wheat.