HvbZIP21, a Novel Transcription Factor From Wild Barley Confers Drought Tolerance by Modulating ROS Scavenging

• DOI: 10.3389/fpls.2022.878459
• Publication Date: 2022-04-22T13:42:34Z
• Authors: Rui Pan; Sebastian Buitrago; Zhenbao Feng; Salah Fatouh Abou...; Le Xu; Chengdao Li; Wenying Zhang
• Keywords: Drought Tolerance

Drought stress is a common environmental stress, which adversely affects the yield and quality of crops. Due to its excellent drought tolerance, wild barley from Middle East region considered valuable source for improvement. Here, we compared growth rate, stomatal regulation capacity metabolize reactive oxygen species (ROS) two cultivars one accession. The results indicated EC_S1 showed more significant decline in aperture less ROS production. Transcriptomic analysis revealed that has slower transcriptional (5,050 DEGs) early stage (14 days) than Baudin (7,022 Tadmor (6,090 DEGs). In addition, 30 hub genes, including nine known drought-related genes were identified by WGCNA analysis. Then, cloned novel bZIP transcription factor, HvbZIP21, EC_S1. HvbZIP21 was subcellularly targeted nucleus. Overexpression Arabidopsis enhanced tolerance due increasing activities superoxide dismutase, peroxidase, catalase as well glutathione content. Silencing suppressed BSMV:HvbZIP21-inoculated plants. Taken together, our findings suggest play critical role manipulating scavenging.