A variable gene family encoding nodule-specific cysteine-rich peptides in pea (Pisum sativum L.)

• DOI: 10.3389/fpls.2022.884726
• Publication Date: 2022-09-14T06:27:10Z
• Authors: Evgeny A. Zorin; Marina S. Kliukova; Alexey M. Afonin; Emma S. Gribchenko; Mikhail L. Gordon; Anton S. Sulima; Aleksandr I. Zher...; Olga A. Kulaeva; Daria A. Romanyuk; Pyotr G. Kusakin; Anna V. Tsyganova; Viktor E. Tsyganov; Igor A. Tikhonovich; Vladimir A. Zhukov
• Keywords: Medicago truncatula; Lotus japonicus

Various legume plants form root nodules in which symbiotic bacteria (rhizobia) fix atmospheric nitrogen after differentiation into a named bacteroids. In some species, bacteroid is promoted by defensin-like nodule-specific cysteine-rich (NCR) peptides. NCR peptides have best been studied the model Medicago truncatula Gaertn., while many other legumes relevant information still fragmentary. Here, we characterize gene family pea ( Pisum sativum L.) using genomic and transcriptomic data. We found 360 genes encoding that are expressed nodules. The sequences of putative highly variable differ significantly from M. . Indeed, only one pair orthologs PsNCR47 – MtNCR312 ) has identified. genome located clusters, expression patterns cluster tend to be similar. These data support idea independent evolution duplication diversification related species. also described spatiotemporal profiles NCRs identified specific transcription factor (TF) binding sites promoters “early” “late” genes. Further, Fix mutants predicted potential regulators expression, among them being TF ERN1 involved early steps nodule organogenesis. general, this study contributes understanding functions diversity antibiotic properties antimicrobial molecules.