Identification of Respiratory Burst Oxidase Homolog (Rboh) Family Genes From Pyropia yezoensis and Their Correlation With Archeospore Release

• DOI: 10.3389/fpls.2022.929299
• Publication Date: 2022-07-12T09:20:34Z
• Authors: Tian-Yu Gui; Da-Hai Gao; Hong-Chang Ding; Xing-Hong Yan
• Keywords: Homology

Reactive oxygen species (ROS) play important regulatory roles in plant growth and development, as well cell differentiation stress responses. Respiratory burst oxidase homolog (RBOH) is the key enzyme ROS production. So far, Rboh family genes Pyropia yezoensis have not been comprehensively characterized, whether their function was involved formation of archeospores still unknown. In this study, a total 11 PyRboh were identified from P. genome by homology mining. Through phylogenetic analysis, it suggested that evolutionarily conserved among lineages red algae, but few exhibited species-specific manner. The treatment blades with NADPH inhibitor diphenylene iodonium (DPI) could significantly inhibit archeospores, suggesting RBOH may be archeospores. According to gene expression analysis using strains obvious differences releasing showed trends most consistent, no significant difference between strains, whereas pattern two yezoensis-specific (PyRbohJ PyRbohK) positively correlated amount Furthermore, allantoin resulted increase release levels PyRbohJ PyRbohK also consistently upregulated, further confirming relationship archeospore formation. These findings provide insights into molecular mechanism