Evolutionary and Integrative Analysis of Gibberellin-Dioxygenase Gene Family and Their Expression Profile in Three Rosaceae Genomes (F. vesca, P. mume, and P. avium) Under Phytohormone Stress

• DOI: 10.3389/fpls.2022.942969
• Publication Date: 2022-07-07T14:46:47Z
• Authors: Irfan Ali Sabir; Muhammad Aamir Ma...; Iftikhar Hussain ...; Farhat Abbas; Xunju Liu; Sajid Fiaz; Adnan Noor Shah; Songtao Jiu; Jiyuan Wang; Muhammad Abdullah; Caixi Zhang
• Keywords: Functional divergence

The gibberellin-dioxygenase (GAox) gene family plays a crucial role in regulating plant growth and development. GAoxs, which are encoded by many subfamilies, extremely critical bioactive GA levels catalyzing the subsequent stages biosynthesis process. Moreover, GAoxs important enzymes synthesis pathway, GAox has not yet been identified Rosaceae species (Prunus avium L., F. vesca, P. mume), especially response to gibberellin PCa (prohexadione calcium; reduce biologically active GAs). In current investigation, 399 members were sweet cherry, Japanese apricot, strawberry. they further classified into six (A-F) subgroups based on phylogeny. According motif analysis structure, majority of PavGAox genes have remarkably well-maintained exon-intron arrangement within same subgroup, may lead functional divergence. systematic several duplication events, but segmental occurs frequently. A calculative orthologous pairs Prunus mume revealed that subjected purifying selection during evolutionary process, resulting cis-regulatory elements upstream region 140 suggests possible relationship between specific functions hormone response-related elements. display variety tissue expression patterns diverse tissues, with most displaying tissue-specific patterns. Furthermore, express significant buds under phytohormonal stresses. Phytohormones stress demonstrated some responsible for maintaining level plant-like Pav co4017001.1 g010.1.br, sc0000024.1 g340.1.br, g270.1.mk. subcellular localization protein utilizing tobacco transient transformation system epidermal cells predicted GFP signals mostly found cytoplasm. These findings will contribute better understanding family's interaction prohexadione calcium GA, as well provide strong framework future characterization cherry.