Transcriptional profiling of long non-coding RNAs regulating fruit cracking in Punica granatum L. under bagging

• DOI: 10.3389/fpls.2022.943547
• Publication Date: 2022-10-11T06:01:07Z
• Authors: Yuying Wang; Yujie Zhao; Yaqiong Wu; Xueqing Zhao; Zhaoxiang Hao; Hua Luo; Zhaohe Yuan
• Keywords: KEGG

Fruit cracking tremendously damages the appearance of fruit, easily leads to pathogen invasion, greatly reduces marketability and causes immense economic losses. The pivotal role long non-coding RNAs (lncRNAs) in diverse biological processes has been confirmed, while roles lncRNAs underlying fruit remain poorly understood. In this study, incidence was 7.26% under bagging treatment, control group 38.11%, indicating that considerably diminished rate. LncRNA libraries for (FC), non-cracking (FNC) (FB) pomegranate ( Punica granatum L.) were performed analysed via high-throughput transcriptome sequencing. A total 3194 obtained with a length 4898846 nt an average 1533.77 pomegranate. We identified 42 differentially expressed (DELs) 137 mRNAs (DEGs) FC vs FNC 35 DELs 160 DEGs FB formed co-expression networks respectively, suggesting there are involved phytohormone signaling pathway, lignin catabolic process, lipid transport/binding, cutin biosynthetic process cell wall organization. also found 18 cis -acting regulated target genes, 10 trans 24 genes FNC, 23 regulate cis-acting 12 36 FC, which provides understanding regulation cracking. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) analysis results demonstrated participated calcium ion binding, glycerophospholipid metabolism, flavonoid biogenesis, xyloglucan metabolic hormone signal transduction starch sucrose metabolism. Our findings provide new insights into regulating lay foundation further improvement quality.