Functional analysis of Pogostemon cablin farnesyl pyrophosphate synthase gene and its binding transcription factor PcWRKY44 in regulating biosynthesis of patchouli alcohol

• DOI: 10.3389/fpls.2022.946629
• Publication Date: 2022-08-26T05:35:32Z
• Authors: Xiaobing Wang; Yun Tang; Huiling Huang; Daidi Wu; Xiuzhen Chen; Junren Li; Hai Zheng; Ruoting Zhan; Likai Chen
• Keywords: Pogostemon; Patchouli; Farnesyl pyrophosphate

Farnesyl pyrophosphate synthase (FPPS) plays an important role in the synthesis of plant secondary metabolites, but its function and molecular regulation mechanism remain unclear Pogostemon cablin . In this study, full-length cDNA FPP gene from P. ( PcFPPS ) was cloned characterized. The expressions are different among tissues (highly flowers). Subcellular localization analysis protoplasts indicated that located cytoplasm. functionally complemented lethal FPPS deletion mutation yeast CC25. Transient overexpression leaves accelerated terpene biosynthesis, with ~47% increase patchouli alcohol. Heterologous tobacco plants achieved, it found enzyme activity significantly up-regulated transgenic by ELISA analysis. addition, more terpenoid including stigmasterol, phytol, neophytadiene were detected compared control GC-MS Furthermore, dual-LUC assay one-hybrid screening, we 220 bp promoter can be bound nuclear-localized transcription factor PcWRKY44. Overexpression PcWRKY44 upregulated expression levels patchoulol PcPTS ), then promote biosynthesis Taken together, these results strongly suggest binding play essential regulating