Comparative transcriptomics analysis of developing peanut (Arachis hypogaea L.) pods reveals candidate genes affecting peanut seed size

• DOI: 10.3389/fpls.2022.958808
• Publication Date: 2022-09-12T08:02:19Z
• Authors: Yue Wu; Ziqi Sun; Feiyan Qi; Mengdi Tian; Juan Wang; Ruifang Zhao; Xiao Wang; Xiaohui Wu; Xinlong Shi; Hongfei Liu; Wenzhao Dong; Bingyan Huang; Zheng Zheng; Xinyou Zhang
• Keywords: Arachis hypogaea; Candidate gene

Pod size is one of the most important agronomic features peanuts, which directly affects peanut yield. Studies on regulation mechanism underpinning pod in cultivated peanuts remain hitherto limited compared to model plant systems. To better understand molecular elements that underpin development, we conducted a comprehensive analysis chronological transcriptomics during development four accessions with similar genetic backgrounds, but varying sizes. Several transcription factors, phytohormones, and mitogen-activated protein kinase (MAPK) signaling pathways were significantly enriched among differentially expressed genes (DEGs) at five consecutive developmental stages, revealing an eclectic range candidate genes, including PNC , YUC IAA regulate auxin synthesis metabolism, CYCD CYCU cell differentiation proliferation, GASA regulates seed elongation via gibberellin pathway. It plausible MPK3 promotes integument division mitotic activity through phosphorylation, interactions between these form network affect size. Furthermore, two variant sites, GCP4 RPPL1 identified are stable QTL interval for attributes function tissue microtubule nucleation. These findings may facilitate identification impart yield improvement peanuts.