Acrophialophora jodhpurensis: an endophytic plant growth promoting fungus with biocontrol effect against Alternaria alternata

• DOI: 10.3389/fpls.2022.984583
• Publication Date: 2022-09-23T14:58:33Z
• Authors: Zoha Daroodi; Parissa Taheri; Saeed Tarighi
• Keywords: 2. Zero hunger; 0303 health sciences; 03 medical and health sciences; Solanum lycopersicum; beneficial endophytic fungus; early blight disease; plant growth parameters; seed coating; Plant culture; Plant Science; SB1-1110

In this study, efficiency of the endophytic fungal isolate Msh5 was evaluated on promoting tomato plant growth and controlling Alternaria alternata, causal agent early blight in tomatoes. Morphological molecular (ITS tub2 sequences) analyses revealed that isolate, Msh5, Acrophialophora jodhpurensis (Chaetomium jodhpurense Lodha). This beneficial fungus capable producing indole-3-acetic acid (IAA), urease, siderophore, extracellular enzymes, solubilized phosphate. Under laboratory conditions, A. inhibited alternata dual culture, volatile non-volatile metabolites assays. The supernatant reducing spore germination altering hyphal structure spores produced germ tubes showed vacuolization abnormal compared to control. Also, effect parameters (such as shoot root weight length) suppressing investigated vivo via seed inoculation with using 1% sugar, 0.5% carboxymethyl cellulose (CMC) or molasses solution stickers. Colonization roots by resulted significant increasing reduction progress diseases caused controls. Among different coating materials used stickers, sugar found be most effective for enhancing decreasing disease progress. Therefore, can suggested a potential biofertilizer biocontrol protecting plants against alternata.