Wood degradation by Fomitiporia mediterranea M. Fischer: Physiologic, metabolomic and proteomic approaches

• DOI: 10.3389/fpls.2022.988709
• Publication Date: 2022-09-26T12:55:06Z
• Authors: Marion Schilling; Alessandra Maia-G...; Raymonde Baltenweck; Emilie Robert; Philippe Hugueney; Christophe Bertsch; Sibylle Farine; Eric Gelhaye
• Keywords: Degradation

Fomitiporia mediterranea (Fmed) is one of the main fungal species found in grapevine wood rot, also called “amadou,” most typical symptoms trunk disease Esca. This fungus functionally classified as a white-rot, able to degrade all structure polymers, i.e., hemicelluloses, cellulose, and recalcitrant component, lignin. Specific enzymes are secreted by those components, namely carbohydrate active for hemicelluloses which can be highly specific given polysaccharide, peroxidases, enable white-rot lignin, with specificities relating lignin composition well. Furthermore, besides diverse set metabolites often associated antifungal activities wood, this differing among various species. Wood decayers possess ability detoxify these extractives could reflect adaptation fungi their environment. The aim study better understand molecular mechanisms used Fmed structure, particular its potential wood. To do so, was cultivated on sawdust from different origins: grapevine, beech, spruce. Carbon mineralization rate, mass loss, polymers contents, targeted (extractives) proteins were measured. We well-known model Trametes versicolor comparison. Whereas no significant degradation observed spruce, higher loss measured culture compared beech culture. Moreover, both substrates, simultaneous pattern demonstrated, proteomic analysis identified relative overproduction oxidoreductases involved extractive cultures, only few differences enzymes. These results explain at least partially structural other species, suggest that biotic abiotic factors should considered fully ecological niche. Proteomics data available via ProteomeXchange identifier PXD036889.