Multi-omics analysis of the regulatory effects of low-phosphorus stress on phosphorus transport in soybean roots

• DOI: 10.3389/fpls.2022.992036
• Publication Date: 2022-09-02T04:51:51Z
• Authors: Hongyu Li; Letian Xu; Jiaxin Li; Xiaochen Lyu; Sha Li; Chang Wang; Xuelai Wang; Chunmei Ma; Chao Yan
• Keywords: Phosphorus deficiency

The regulatory effects of uneven phosphorus supplies on transport in soybean roots are still unclear. To further analyze the low-phosphorus stress and application physiological mechanism roots, dual-root plants were prepared via grafting, a sand culture experiment was performed. From unfolded cotyledon stage to initial flowering stage, one side each system irrigated with low-phosphorus-concentration solution (phosphorus-application [P+] side), other phosphorus-free nutrient (phosphorus-free [P-] side); this setup allowed study different supply levels expression genes proteins accumulation metabolites P- clarify method through which is regulated provide theoretical basis for improving use rate fertilizer. results revealed that unilateral low-concentration promoted uptake by from P+ side. Compared normal concentration supply, low affected regulation metabolic pathways involved starch sucrose metabolism, glycolysis, fructose, mannose etc., thereby affecting root transport. inhibited fructose synthesis synthase hexokinase (HK) kinase, catalyzes conversion fructose-6-phosphate. Low-phosphorus invertase into maltose activity (StS) stimulated UDPG pyrophosphorylase (UGP) phosphoglucose isomerase (GP1), UDP-glucose glucose-6-phosphate. pathway then affected, allocation UTP Additionally, hastened glycolysis malic acid, promoting roots. In addition, mannose, mannose-1-phosphate enzymes as well invertase, inhibiting several organic phosphorus-containing compounds.