Genome-wide characterization and identification of Trihelix transcription factors and expression profiling in response to abiotic stresses in Chinese Willow (Salix matsudana Koidz)

• DOI: 10.3389/fpls.2023.1125519
• Publication Date: 2023-03-03T05:38:35Z
• Authors: Jie Yang; Zhixuan Tang; Wuyue Yang; Qianhui Huang; Yuqing Wang; Mengfan Huang; Hui Wei; Guoyuan Liu; Bolin Lian; Yanhong Chen; Jian Zhang
• Keywords: Salix matsudana; genome-wide characterization; Trihelix family; Plant culture; submergence stress; RNA-Seq; Plant Science; 15. Life on land; SB1-1110

Trihelix transcription factors (TTF) are a class of light-responsive proteins with typical triple-helix structure (helix-loop-helix-loop-helix). Members this gene family play an important role in plant growth and development, especially various abiotic stress responses. Salix matsudana Koidz is allotetraploid ornamental forest tree that widely planted for its excellent resistance to stress, but no studies on have been reported. In study, the was analyzed at genome-wide level S. . A total 78 ( SmTTFs ) were identified, distributed 29 chromosomes, classified into four subfamilies (GT-1, GT-2, SH4, SIP1) based their structural features. The structures conserved functional domains these genes similar same subfamily differ between subfamilies. presence multiple stress-responsive cis-elements promoter suggests may respond stresses. Expression pattern analysis revealed different functions during flooding salt drought low temperature Given SmTTF30 , as differentially expressed gene, has faster response we selected studies. Overexpression Arabidopsis thaliana (Arabidopsis) enhances tolerance stress. Under leaf cell activity peroxidase (POD) overexpression strain significantly higher than POD wild type, malondialdehyde (MDA) content lower MDA type. Thus, results suggest plays positive regulatory tolerance.