Analysis of the molecular and biochemical mechanisms involved in the symbiotic relationship between Arbuscular mycorrhiza fungi and Manihot esculenta Crantz

• DOI: 10.3389/fpls.2023.1130924
• Publication Date: 2023-03-07T05:39:12Z
• Authors: Yu Gao; Siyuan Huang; Yujie Wang; Hongxin Lin; Zhiyong Pan; Shubao Zhang; Jie Zhang; Wenquan Wang; Shanhan Cheng; Yinhua Chen
• Keywords: Arbuscular mycorrhiza; Manihot esculenta; Arbuscular mycorrhizal fungi; Mycorrhizal Fungi; Glomeromycota

Plants and arbuscular mycorrhizal fungi (AMF) mutualistic interactions are essential for sustainable agriculture production. Although it is shown that AMF inoculation improves cassava physiological performances yield traits, the molecular mechanisms involved in AM symbiosis remain largely unknown. Herein, we integrated metabolomics transcriptomics analyses of symbiotic (Ri) asymbiotic (CK) roots explored AM-induced biochemical transcriptional changes.Three weeks (3w) after inoculations, proliferating fungal hyphae were observable, plant height root length significantly increased. In total, identified 1,016 metabolites, which 25 differentially accumulated (DAMs) at 3w. The most highly induced metabolites 5-aminolevulinic acid, L-glutamic lysoPC 18:2. Transcriptome analysis 693 6,481 expressed genes (DEGs) comparison between CK against Ri 3w 6w, respectively. Functional enrichment DAMs DEGs unveiled transport, amino acids sugar metabolisms, biosynthesis secondary hormone signal transduction, phenylpropanoid biosynthesis, plant-pathogen as regulated pathways. Potential candidate genes, including nitrogen phosphate transporters, transcription factors, phytohormone, metabolism-related, SYM (symbiosis) signaling pathway-related, future functional studies.Our results provide insights into valuable resources improving