Overexpression of GmWRKY172 enhances cadmium tolerance in plants and reduces cadmium accumulation in soybean seeds

• DOI: 10.3389/fpls.2023.1133892
• Publication Date: 2023-03-09T05:33:05Z
• Authors: Peiqi Xian; Yuan Yang; Chuwen Xiong; Zhibin Guo; Intikhab Alam; Zihang He; Yakun Zhang; Zhandong Cai; Hai Nian
• Keywords: WRKY protein domain

Introduction Cadmium (Cd) stress is a significant threat to soybean production, and enhancing Cd tolerance in the focus of this study. The WRKY transcription factor family associated with abiotic response processes. In study, we aimed identify Cd-responsive GmWRKY172 from investigate its potential for soybean. Methods characterization involved analyzing expression pattern, subcellular localization, transcriptional activity. To assess impact , transgenic Arabidopsis plants were generated examined their content shoots. Additionally, evaluated translocation various physiological indicators. RNA sequencing was performed biological pathways regulated by GmWRKY172. Results significantly upregulated stress, highly expressed leaves flowers, localized nucleus Transgenic overexpressing showed enhanced reduced shoots compared WT. Lower roots seeds also observed Under accumulated less malondialdehyde (MDA) hydrogen peroxide (H 2 O ) than WT plants, higher flavonoid lignin contents, peroxidase (POD) analysis revealed that many stress-related soybean, including biosynthesis, cell wall synthesis, Discussion Our findings demonstrated enhances reduces seed accumulation regulating multiple pathways, could be promising candidate breeding Cd-tolerant low varieties.