Identification of major QTLs for yield-related traits with improved genetic map in wheat

• DOI: 10.3389/fpls.2023.1138696
• Publication Date: 2023-03-17T05:00:35Z
• Authors: Feifei Ma; Yunfeng Xu; Ruifang Wang; Yiping Tong; Aimin Zhang; Dongcheng Liu; Diaoguo An
• Keywords: Identification; Genetic linkage map

Introduction Identification of stable major quantitative trait loci (QTLs) for yield-related traits is important yield potential improvement in wheat breeding. Methods In the present study, we genotyped a recombinant inbred line (RIL) population using Wheat 660K SNP array and constructed high-density genetic map. The map showed high collinearity with genome assembly. Fourteen were evaluated six environments QTL analysis. Results Discussion A total 12 environmentally QTLs identified at least three environments, explaining up to 34.7% phenotypic variation. Of these, QTkw-1B.2 thousand kernel weight (TKW), QPh-2D.1 ( QSl-2D.2/QScn-2D.1 ) plant height (PH), spike length (SL) spikelet compactness (SCN), QPh-4B.1 PH, QTss-7A.3 number per (TSS) detected five environments. set Kompetitive Allele Specific PCR (KASP) markers converted based on above used genotype diversity panel comprising 190 accessions across four growing seasons. ), successfully validated. Compared previous studies, should be novel QTLs. These results provided solid foundation further positional cloning marker-assisted selection targeted breeding programs.