High-quality Momordica balsamina genome elucidates its potential use in improving stress resilience and therapeutic properties of bitter gourd

• DOI: 10.3389/fpls.2023.1258042
• Publication Date: 2024-01-24T04:32:41Z
• Authors: N. D. Vinay; Kalpana Singh; Ranjith Kumar Ellur; Viswanathan Chinn...; Sarika Jaiswal; Mir Asif Iquebal; Anilabha Das Munshi; Hideo Matsumura; G. Boopalakrishnan; Gograj Singh Jat; Chittaranjan Kole; Ambika Baladev Ga...; Dinesh Kumar; Shyam Sundar Dey; Tusar Kanti Behera
• Keywords: 0301 basic medicine; 03 medical and health sciences; cucurbits; stress tolerance; medicinal value; genome assembly; Plant culture; Plant Science; genomic resources; Momordica balsamina; SB1-1110

IntroductionMomordica balsamina is the closest wild species that can be crossed with an important fruit vegetable crop, Momordica charantia, has immense medicinal value, and placed under II subclass of primary gene pool of bitter gourd. M. balsamina is tolerant to major biotic and abiotic stresses. Genome characterization of Momordica balsamina as a wild relative of bitter gourd will contribute to the knowledge of the gene pool available for improvement in bitter gourd. There is potential to transfer gene/s related to biotic resistance and medicinal importance from M. balsamina to M. charantia to produce high-quality, better yielding and stress tolerant bitter gourd genotypes.MethodsThe present study provides the first and high-quality chromosome-level genome assembly of M. balsamina with size 384.90 Mb and N50 30.96 Mb using sequence data from 10x Genomics, Nanopore, and Hi-C platforms.ResultsA total of 6,32,098 transposons elements; 2,15,379 simple sequence repeats; 5,67,483 transcription factor binding sites; 3,376 noncoding RNA genes; and 41,652 protein-coding genes were identified, and 4,347 disease resistance, 67 heat stress–related, 05 carotenoid-related, 15 salt stress–related, 229 cucurbitacin-related, 19 terpenes-related, 37 antioxidant activity, and 06 sex determination–related genes were characterized.ConclusionGenome sequencing of M. balsamina will facilitate interspecific introgression of desirable traits. This information is cataloged in the form of webgenomic resource available at http://webtom.cabgrid.res.in/mbger/. Our finding of comparative genome analysis will be useful to get insights into the patterns and processes associated with genome evolution and to uncover functional regions of cucurbit genomes.