High-quality Momordica balsamina genome elucidates its potential use in improving stress resilience and therapeutic properties of bitter gourd

• DOI: 10.3389/fpls.2023.1258042
• Publication Date: 2024-01-24T04:32:41Z
• Authors: N. D. Vinay; Kalpana Singh; Ranjith Kumar Ellur; Viswanathan Chinn...; Sarika Jaiswal; Mir Asif Iquebal; Anilabha Das Munshi; Hideo Matsumura; G. Boopalakrishnan; Gograj Singh Jat; Chittaranjan Kole; Ambika Baladev Ga...; Dinesh Kumar; Shyam Sundar Dey; Tusar Kanti Behera
• Keywords: Bitter gourd; Gourd; Biotic stress

Introduction Momordica balsamina is the closest wild species that can be crossed with an important fruit vegetable crop, charantia , has immense medicinal value, and placed under II subclass of primary gene pool bitter gourd. M. tolerant to major biotic abiotic stresses. Genome characterization as a relative gourd will contribute knowledge available for improvement in There potential transfer gene/s related resistance importance from produce high-quality, better yielding stress genotypes. Methods The present study provides first high-quality chromosome-level genome assembly size 384.90 Mb N50 30.96 using sequence data 10x Genomics, Nanopore, Hi-C platforms. Results A total 6,32,098 transposons elements; 2,15,379 simple repeats; 5,67,483 transcription factor binding sites; 3,376 noncoding RNA genes; 41,652 protein-coding genes were identified, 4,347 disease resistance, 67 heat stress–related, 05 carotenoid-related, 15 salt 229 cucurbitacin-related, 19 terpenes-related, 37 antioxidant activity, 06 sex determination–related characterized. Conclusion sequencing facilitate interspecific introgression desirable traits. This information cataloged form webgenomic resource at http://webtom.cabgrid.res.in/mbger/ . Our finding comparative analysis useful get insights into patterns processes associated evolution uncover functional regions cucurbit genomes.