Insight into regulation of adventitious root formation by arbuscular mycorrhizal fungus and exogenous auxin in tea plant (Camellia sinensis L.) cuttings

• DOI: 10.3389/fpls.2023.1258410
• Publication Date: 2023-09-19T07:31:57Z
• Authors: Weili Chen; Wenshu Shan; Tingting Niu; Tao Ye; Qinyu Sun; Jiaxia Zhang
• Keywords: Cutting; Herbaceous plant

Introduction Adventitious root (AR) development, affected by various biotic and abiotic factors, is the most important procedure in tea plant ( Camellia sinensis L.) cutting propagation. Establishing symbiotic relationships with terrestrial plants, AMF (Arbuscular mycorrhizal fungus) can mediate AR formation of several herbaceous woody plants previous studies. Methods In this paper, effects combined application exogenous auxin on cuttings from different varieties (‘Pingyangtezao’, ‘Longjing 43’ ‘Longjingchangye’) were studied. Then we also performed RNA-Seq analysis ‘Pingyangtezao’ aiming to find possible auxin-related pathway AM fungal regulation formation. To accurately uncover regulatory mechanism cuttings, rooting process separated into four stages (S0, non-rooting; S1, protrusion; S2, S3, elongation) at same sampling time. Results Discussion showed that IBA treatment increased colonization rate, especially variety (from 37.58% 46.29%). Both inoculating addition promoted formation, dependence auxin. could alleviate effect inhibitors (2,3,5-triiodobenzoic acid, L-α-(Aminooxy)-β-phenylpropionic acid α-(phenylethyl-2-oxo)-IAA) even though was hindered degrees. Transcriptomic numbers differentially expressed genes (DEGs) S2 stage (1360 DEGs), indicating increasing development AR. Similar trend found DEGs, family YUC , GH PIN LAX SAUR AUX ABP involved cuttings. Additionally, strongly mediated transport signal transduction pathways as results correlation analysis. Overall, interaction promoting preliminary regulating deciphered which may provide a basis for further deep mechanistic research propagation production.