Differential gene expression and potential regulatory network of fatty acid biosynthesis during fruit and leaf development in yellowhorn (Xanthoceras sorbifolium), an oil-producing tree with significant deployment values

• DOI: 10.3389/fpls.2023.1297817
• Publication Date: 2024-01-19T04:26:03Z
• Authors: Tian-Le Shi; Hai-Yao Ma; Xinrui Wang; Hui Liu; Xue-Mei Yan; Xue-Chan Tian; Zhi-Chao Li; Yu-Tao Bao; Zhao-Yang Chen; Shi-Wei Zhao; Qiuhong Xiang; Kai-Hua Jia; Shuai Nie; Wenbin Guan; Jian-Feng Mao
• Keywords: MYB; Metabolic pathway; Gene regulatory network; Metabolic Engineering

Xanthoceras sorbifolium (yellowhorn) is a woody oil plant with super stress resistance and excellent characteristics. The yellowhorn can be used as biofuel edible high nutritional medicinal value. However, genetic studies on are just in the beginning, fundamental biological questions regarding its very long-chain fatty acid (VLCFA) biosynthesis pathway remain largely unknown. In this study, we reconstructed VLCFA annotated 137 genes encoding relevant enzymes. We identified four oleosin that package triacylglycerols (TAGs) specifically expressed fruits, likely playing key roles production. Especially, by examining time-ordered gene co-expression network (TO-GCN) constructed from fruit leaf developments, enzymatic potential regulatory transcription factors involved synthesis. further inferred hierarchical MYB-related ( XS03G0296800 ) B3 XS02G0057600 top-tier regulators, providing clues into controlling carbon flux acids. Our results offer new insights transcriptional regulators governing production yellowhorn, laying foundation for efforts to optimize content composition. Moreover, expression patterns putative relationships here will inform metabolic engineering molecular breeding approaches tailored meet bioproduct demands.